Neutral polysaccharide from Panax notoginseng enhanced cyclophosphamide antitumor efficacy in hepatoma H22-bearing mice

Abstract Background Our previous studies demonstrated that the administration of crude Polysaccharide from Panax notoginseng (CPPN) can effectively prolong lifespan tumor-bearing mice via boosting host immune system as well weak cytotoxicity against hepatocellular carcinoma (HCC). In present study, Neutral (NPPN) were further purified polysaccharide isolated panax . The effects NPPN on function and hematopoietic with low immunity myelosuppression induced by cyclophosphamide (CTX) investigated. effect combined CTX tumor inhibition rate H22 impact proliferation liver cancer cells in vitro Methods CPPN was obtained water extraction alcohol precipitation method, DEAE Sepharose Fast Flow ion exchange resin column. added to immunosuppressed CTX. Thymus index, spleen lymphocyte stimulation index adding concanavalin A, determination serum hemolysin, NK cell activity assay, carbon clearance experiment, blood count tests detected. treated recorded. Results 4 kinds acid (APPN) successfully inhibited growth significantly increase elevation cellular humoral levels a variety indicators immunosuppressive may contribute antitumor NPPN. Conclusion has potential for treatment cyclophosphamide.